Liquid Handling and the ELISA Protocol: Making the case for a 96-channel semi-automated pipette in your lab

Abstract: Enzyme-linked immunosorbent assays (ELISA) are commonly used analytical biochemistry assays. The specific techniques vary significantly in the details and sequence of the biological interactions that occur, but they all require a similar range of liquid handling operations. The purpose of this paper is to illuminate the potential benefits of using the Avidien microPro300 semi-automated benchtop pipettor when performing these assays in either a 96 or 384 well microplate format.

1 Introduction
Enzyme-linked immunosorbent assays (ELISA) are commonly used analytical biochemistry assays, and for that reason we have chosen a generic ELISA protocol to demonstrate a few of the benefits and time savings that can be realized by using a 96-channel semi-automated benchtop pipette. Frequently used ELISA methods include direct, indirect, sandwich and competition assays. These techniques vary significantly in the details and sequence of the biological interactions that occur, but they all require a similar range of liquid handling operations. The purpose of this paper is to illuminate the potential benefits of using the Avidien microPro300 semi-automated benchtop pipette when performing these assays in either 96 or 384 well microplate formats.

Learn about the Liquid handling steps required in successfully executing a typical ELISA: Click here to read more